PacBio, third generation DNA sequencing

PacBio RS II

CAT-AgroFood sequencing facilities offer the PacBio RS II system, a third generation DNA sequencer that provides analysis of Single Molecule in Real Time (SMRT).

PacBio RS II with SMRT technology is able to generate very long reads from single DNA molecules without prior DNAamplification. These multiple kilobases long sequence reads are essential for assembly of large and complex genomes or discovery of large structural variations.

Key advantages of the PacBio RS II over other NGS platforms include:

  • Single molecule real time detection
  • Long reads (average > 10 Kb)
  • Amplification free sample preparation