Illumina HiSeq2500


The Illumina HiSeq2500 with v4 chemistry is based on the concept of ‘sequencing by synthesis’ to produce sequence reads up to 250 base pairs long derived from billions of surface-amplified DNA fragments simultaneously.

Depending on user specified settings, reads can be 125 or 250 base pairs long and derived from single read or paired end read chemistry, with a maximum throughput of 1 Tera bases per run.

Key advantages of the HiSeq2500 over other NGS platforms include:

  • Highest throughput
  • Lowest run cost/base
  • Best-established technology
  • Broadest range of biological applications